Dynamic cytotoxic profiles of sulfur mustard in human dermal cells determined by multiparametric high-content analysis.

ABSTRACT: Sulfur mustard (SM) is a well known chemical warfare agent that poses a major threat to military personnel and also populace. It targets multiple macromolecules, and its toxic effects are mediated by complex mechanisms. However, the sequence and manner of SM-induced cellular and molecular events underpinning the pathological processes are not fully elucidated. Effective therapeutic agents against SM poisoning are also lacking. The present study aimed to determine the dynamic cytotoxic profiles of SM in primary cultured human epidermal keratinocytes-fetal (HEK-f) and human dermal fibroblasts-adult (HDF-a) by establishing a high content analysis (HCA)-based multiparametric toxicity assay panel. SM was found to produce multiple, concentration-dependent cellular responses, including abnormal c

SUBMITTER: Long L 

PROVIDER: S-EPMC6062398 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature