Production of biologically active feline interferon beta in insect larvae using a recombinant baculovirus.

ABSTRACT: Feline interferon beta is a cytokine that belongs to the type I IFN family, with antitumor, antiviral and immunomodulatory functions. In this work, recombinant feline interferon beta (rFeIFN?) was expressed in insect larvae that constitute important agronomic plagues. rFeIFN? accumulated in the hemolymph of Spodoptera frugiperda larvae infected with recombinant baculovirus and was purified by Blue-Sepharose chromatography directly from larval homogenates on day 4 post-infection. rFeIFN? was recovered after purification with a specific activity of 1?×?106 IU mg-1. By this method, we obtained 8.9?×?104 IU of purified rFeIFN? per larva. The product was biologically active in vitro, with an antiviral activity of 9.5?×?104 IU mL-1, as well as a potent antitumor activity comparable to that of the commercial FeIFN?. The glycosylation of rFeIFN? was confirmed by peptide-N-glycosidase F digestion. Our findings provide a cost-effective platform for large-scale rFeIFN? production in laboratory research or veterinary medicine applications.

SUBMITTER: Arregui MB 

PROVIDER: S-EPMC6063813 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature