Ontology highlight
ABSTRACT:
SUBMITTER: Johns NI
PROVIDER: S-EPMC6065261 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Johns Nathan I NI Gomes Antonio L C ALC Yim Sung Sun SS Yang Anthony A Blazejewski Tomasz T Smillie Christopher S CS Smith Mark B MB Alm Eric J EJ Kosuri Sriram S Wang Harris H HH
Nature methods 20180319 5
Robust and predictably performing synthetic circuits rely on the use of well-characterized regulatory parts across different genetic backgrounds and environmental contexts. Here we report the large-scale metagenomic mining of thousands of natural 5' regulatory sequences from diverse bacteria, and their multiplexed gene expression characterization in industrially relevant microbes. We identified sequences with broad and host-specific expression properties that are robust in various growth conditi ...[more]