Dataset Information

Fouling Development in A/O-MBR under Low Organic Loading Condition and Identification of Key Bacteria for Biofilm Formations.

ABSTRACT: Membrane fouling in membrane bioreactors (MBR) remains a major issue and knowledge of microbes associated with biofilm formation might facilitate the control of this phenomenon, Thus, an anoxic/oxic membrane bioreactor (A/O-MBR) was operated under an extremely low organic loading rate (0.002 kg-COD·m^-3·day^-1) to induce membrane fouling and the major biofilm-forming bacteria were identified. After operation under extremely low organic loading condition, the reactor showed accumulation of total nitrogen and phosphorus along with biofilm development on the membrane surface. Thus, membrane fouling induced by microbial cell lysis was considered to have occurred. Although no major changes were observed in the microbial community structure of the activated sludge in the MBR before and after membrane fouling, uncultured bacteria were specifically increased in the biofilm. Therefore, bacteria belonging to candidate phyla including TM6, OD1 and Gammaproteobacteria could be important biofilm-forming bacteria.

SUBMITTER: Takimoto Y

PROVIDER: S-EPMC6065318 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Fouling Development in A/O-MBR under Low Organic Loading Condition and Identification of Key Bacteria for Biofilm Formations.

Takimoto Yuya Y Hatamoto Masashi M Ishida Takaya T Watari Takahiro T Yamaguchi Takashi T

Scientific reports 20180730 1

Membrane fouling in membrane bioreactors (MBR) remains a major issue and knowledge of microbes associated with biofilm formation might facilitate the control of this phenomenon, Thus, an anoxic/oxic membrane bioreactor (A/O-MBR) was operated under an extremely low organic loading rate (0.002 kg-COD·m<sup>-3</sup>·day<sup>-1</sup>) to induce membrane fouling and the major biofilm-forming bacteria were identified. After operation under extremely low organic loading condition, the reactor showed ac ...[more]

PMID: 30061582

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Project description:In the food industry, especially dairy, biofilms can be formed by heat-resistant spoilage and pathogenic bacteria from the farm. Such biofilms may persist throughout the processing chain and contaminate milk and dairy products continuously, increasing equipment cleaning, maintenance costs, and product recalls. Most biofilms are multispecies, yet most studies focus on single-species models. A multispecies model of dairy biofilm was developed under static and dynamic conditions using heat-resistant Bacillus licheniformis, Pseudomonas aeruginosa, Clostridium tyrobutyricum, Enterococcus faecalis, Streptococcus thermophilus, and Rothia kristinae isolated from dairies. C. tyrobutiricum and R. kristinae were weak producers of biofilm, whereas the other four were moderate to strong producers. Based on cross-streaking on agar, P. aeruginosa was found to inhibit B. licheniformis and E. faecalis. In multispecies biofilm formed on stainless steel in a CDC reactor fed microfiltered milk, the strong biofilm producers were dominant while the weak producers were barely detectable. All biofilm matrices were dispersed easily by proteinase K treatment but were less sensitive to DNase or carbohydrases. Further studies are needed to deepen our understanding of multispecies biofilms and interactions within to develop improved preventive strategies to control the proliferation of spoilage and pathogenic bacteria in dairies and other food processing environments. IMPORTANCE A model of multispecies biofilm was created to study biofilm formation by heat-resistant bacteria in the dairy industry. The biofilm formation potential was evaluated under static conditions. A continuous flow version was then developed to study multispecies biofilm formed on stainless steel in microfiltered milk under dynamic conditions encountered in dairy processing equipment. The study of biofilm composition and bacterial interactions therein will lead to more effective means of suppressing bacterial growth on food processing equipment and contamination of products with spoilage and pathogenic bacteria, which represent considerable economic loss.

Dataset Information

Fouling Development in A/O-MBR under Low Organic Loading Condition and Identification of Key Bacteria for Biofilm Formations.

Publications

Fouling Development in A/O-MBR under Low Organic Loading Condition and Identification of Key Bacteria for Biofilm Formations.

Similar Datasets

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