Dataset Information

Mycobacterium Growth Inhibition Assay of Human Alveolar Macrophages as a Correlate of Immune Protection Following Mycobacterium bovis Bacille Calmette-Guerin Vaccination.

ABSTRACT:

Background

In order to eliminate tuberculosis (TB), an effective vaccine is urgently needed to prevent infection with Mycobacterium tuberculosis. A key obstacle for the development of novel TB vaccines is the lack of surrogate markers for immune protection against M. tuberculosis.

Methods

We investigated growth rates of M. tuberculosis in the mycobacterial growth inhibition assay (MGIA) as a marker for mycobacterial growth control of human bronchoalveolar lavage (BALC) and peripheral blood mononuclear cells (PBMC) before and after vaccination with Mycobacterium bovis Bacille Calmette-Guérin (BCG) of healthy adult volunteers.

Results

Vaccination induced a positive response (p?M. bovis BCG-vaccination revealed no significant difference in time to culture positivity before and after vaccination in BALC (p?=?0.604) and PBMC (p?=?0.199). The magnitude of the PPD-response induced by M. bovis BCG-vaccination did not correlate with growth control in BALC and PBMC (correlation?=?0.468, 95% CI: -0.016 to 0.775).

Conclusion

In conclusion, M. bovis BCG-vaccination-induced mycobacterial-specific cytokine immune response does not result in functional immune control against M. tuberculosis in the MGIA.

SUBMITTER: Radloff J

PROVIDER: S-EPMC6066571 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

<i>Mycobacterium</i> Growth Inhibition Assay of Human Alveolar Macrophages as a Correlate of Immune Protection Following Mycobacterium bovis Bacille Calmette-Guérin Vaccination.

Radloff Juliane J Heyckendorf Jan J van der Merwe Lize L Sanchez Carballo Patricia P Reiling Norbert N Richter Elvira E Lange Christoph C Kalsdorf Barbara B

Frontiers in immunology 20180724

<h4>Background</h4>In order to eliminate tuberculosis (TB), an effective vaccine is urgently needed to prevent infection with <i>Mycobacterium tuberculosis</i>. A key obstacle for the development of novel TB vaccines is the lack of surrogate markers for immune protection against <i>M. tuberculosis</i>.<h4>Methods</h4>We investigated growth rates of <i>M. tuberculosis</i> in the mycobacterial growth inhibition assay (MGIA) as a marker for mycobacterial growth control of human bronchoalveolar lava ...[more]

PMID: 30087678

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