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Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells.

ABSTRACT: We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes' expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.

SUBMITTER: Yu L

PROVIDER: S-EPMC6067523 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells.

Yu Leqian L Li Junjun J Hong Jiayin J Takashima Yasuhiro Y Fujimoto Nanae N Nakajima Minako M Yamamoto Akihisa A Dong Xiaofeng X Dang Yujiao Y Hou Yu Y Yang Wei W Minami Itsunari I Okita Keisuke K Tanaka Motomu M Luo Chunxiong C Tang Fuchou F Chen Yong Y Tang Chao C Kotera Hidetoshi H Liu Li L

Stem cell reports 20180628 1

We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes' expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response f ...[more]

PMID: 30008324

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Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

Project description:The human pluripotent stem cells cultured on Low-adhesion substrates formed two morphologically different subtypes (Monolayer and Domed). The dome-like cells showed higher proliferation capacity and KLF4/5 expression than the monolayer cells. A serum response factor based regulatory double loop was proposed to explain how cell-matrix adhesion mediates the interaction between cell morphology and pluripotency genes

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