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Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for Neospora caninum and Toxoplasma gondii and Its Immune Response in an Animal Model.

ABSTRACT: Background:We aimed to construct an adenovirus expressing a cross-reactive fragment of the apical membrane antigen 1 (AMA1) antigen and evaluated the concomitant immune response in BABL/c mice, allowing protection against N. caninum and T. gondii infection. Methods:The study was conducted in Agricultural College of Yanbian University, Yanji, Jilin, China In 2015-2016. Primers were designed using the AMA1 gene sequences of N. caninum (AB265823.1) and T. gondii (AF010264.1). After linearization of the plasmid ADV4-NcAMA1 and the framework plasmid pacAd5, a total of 293T cells were cotransfected and Ad5-NcAMA1 recombinant adenovirus were packed. BALB/c mice were inoculated. Simultaneously serum IgG antibody levels and IFN-? and IL-4 cytokine levels were determined by ELISA. After immunization three times in two weeks, each group of BABL/c mice were divided into two groups, respectively given intraperitoneal inoculation by the Neospora tachyzoite and Toxoplasma tachyzoite. Then we observed the clinical symptoms and statistical survival rate of mice. Results:The level of IgG in BABL/c mice immunized with Ad5-NcAMA1 was significantly increased when compared with that of pVAX1-NcAMA1 and PBS groups (P<0.01). At the same time, the cytokine levels of IFN-? and IL-4 were also higher in the Ad4-NcAMA1 group than in the control groups (P<0.01). Moreover, BABL/c mice immunized with Ad5-NcAMA1, pVAX1-NcAMA1, and PBS showed survival rates of 75%, 45% and 20% after N. caninum infection, and 45%, 10% and 0% after T. gondii infection, respectively. Conclusion:The adenovirus vaccineAd5-NcAMA1 could provide protective immunity against N. caninum and T. gondii infection.

SUBMITTER: Jia L

PROVIDER: S-EPMC6068368 | biostudies-literature | 2018 Apr-Jun

REPOSITORIES: biostudies-literature

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Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for <i>Neospora caninum</i> and <i>Toxoplasma gondii</i> and Its Immune Response in an Animal Model.

Jia Lijun L Guo Huanping H Liu Mingming M Gao Yang Y Zhang Lei L Li Hang H Xie Suzhu S Zhang Ningning N

Iranian journal of parasitology 20180401 2

<h4>Background</h4>We aimed to construct an adenovirus expressing a cross-reactive fragment of the apical membrane antigen 1 (AMA1) antigen and evaluated the concomitant immune response in BABL/c mice, allowing protection against <i>N. caninum</i> and <i>T. gondii</i> infection.<h4>Methods</h4>The study was conducted in Agricultural College of Yanbian University, Yanji, Jilin, China In 2015-2016. Primers were designed using the AMA1 gene sequences of <i>N. caninum</i> (AB265823.1) and <i>T. gond ...[more]

PMID: 30069207

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Project description:BACKGROUND:Knowledge about parasitic infections is crucial information for animal health, particularly of free-ranging species that might come into contact with livestock and humans. METHODS:We investigated the seroprevalence of three tissue-cyst-forming apicomplexan parasites (Toxoplasma gondii, Neospora caninum and Besnoitia besnoiti) in 506 individuals of 12 wildlife species in Namibia using in-house enzyme linked immunosorbent assays (indirect ELISAs applying purified antigens) for screening and immunoblots as confirmatory tests. We included six species of the suborder Feliformia, four species of the suborder Caniformia and two species of the suborder Ruminantia. For the two species for which we had most samples and life-history information, i.e. cheetahs (Acinonyx jubatus, n?=?250) and leopards (Panthera pardus, n?=?58), we investigated T. gondii seroprevalence in relation to age class, sex, sociality (solitary, mother-offspring group, independent sibling group, coalition group) and site (natural habitat vs farmland). RESULTS:All but one carnivore species (bat-eared fox Otocyon megalotis, n?=?4) were seropositive to T. gondii, with a seroprevalence ranging from 52.4% (131/250) in cheetahs to 93.2% (55/59) in African lions (Panthera leo). We also detected antibodies to T. gondii in 10.0% (2/20) of blue wildebeest (Connochaetes taurinus). Adult cheetahs and leopards were more likely to be seropositive to T. gondii than subadult conspecifics, whereas seroprevalence did not vary with sex, sociality and site. Furthermore, we measured antibodies to N. caninum in 15.4% (2/13) of brown hyenas (Hyaena brunnea) and 2.6% (1/39) of black-backed jackals (Canis mesomelas). Antibodies to B. besnoiti were detected in 3.4% (2/59) of African lions and 20.0% (4/20) of blue wildebeest. CONCLUSIONS:Our results demonstrate that Namibian wildlife species were exposed to apicomplexan parasites at different prevalences, depending on parasite and host species. In addition to serological work, molecular work is also needed to better understand the sylvatic cycle and the clear role of wildlife in the epidemiology of these parasites in southern Africa.

Dataset Information

Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for Neospora caninum and Toxoplasma gondii and Its Immune Response in an Animal Model.

Publications

Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for <i>Neospora caninum</i> and <i>Toxoplasma gondii</i> and Its Immune Response in an Animal Model.

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