Dataset Information

MicroRNA?92a?3p inhibits the cell proliferation, migration and invasion of Wilms tumor by targeting NOTCH1.

ABSTRACT: Dysregulation of miR?92a?3p has been shown to contribute to many tumorigenic processes, and is correlated with tumor progression and prognosis. However, the association between miR?92a?3p and the clinicopathological features of Wilms tumorand its regulatory mechanism remain unknown. In the present study, we demonstrated that miR?92a?3p was downregulated in Wilms tumor tissues and was signi?cantly correlated with the lung metastasis of patients with Wilms tumor. Furthermore, miR?92a?3p mimics suppressed Wilms tumor cell proliferation, migration and invasion by in vitro assays. In addition, miR?92a?3p knockdown promoted tumor progression. Moreover, miR?92a?3p was shown to target directly the 3'?UTR of NOTCH1 mRNA by Dual?Luciferase reporter assays in Wilm's tumor cells. miR?92a?3p mimics decreased the expression of mRNA and protein of NOTCH1. miR?92a?3p inhibitor enhanced NOTCH1 expression by using western blotting and qPCR. In Wilms tumor tissues, NOTCH1 was highly expressed when compared with that in adjacent non?tumor tissues. NOTCH1 expression was found to be negatively correlated with miR?92a?3p in tumor tissues. Knockdown of NOTCH1 expression reversed the promotive effect of miR?92a?3p inhibitor on the cell proliferation, migration and invasion in Wilms tumor. In conclusion, miR?92a?3p blocks the progression of Wilms tumor by targeting NOTCH1.

SUBMITTER: Zhu S

PROVIDER: S-EPMC6072285 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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MicroRNA‑92a‑3p inhibits the cell proliferation, migration and invasion of Wilms tumor by targeting NOTCH1.

Zhu Shibo S Zhang Liyu L Zhao Zhang Z Fu Wen W Fu Kai K Liu Guochang G Jia Wei W

Oncology reports 20180523 2

Dysregulation of miR‑92a‑3p has been shown to contribute to many tumorigenic processes, and is correlated with tumor progression and prognosis. However, the association between miR‑92a‑3p and the clinicopathological features of Wilms tumorand its regulatory mechanism remain unknown. In the present study, we demonstrated that miR‑92a‑3p was downregulated in Wilms tumor tissues and was signiﬁcantly correlated with the lung metastasis of patients with Wilms tumor. Furthermore, miR‑92a‑3p mimics sup ...[more]

PMID: 29845267

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Project description:BackgroundIncreasing evidence has suggested that dysregulation of certain microRNAs (miRNAs) may contribute to human disease including carcinogenesis and tumor metastasis in human. miR-124-3p is down-regulated in various cancers, and modulates proliferation and aggressiveness of cancer cells. However, the roles of miR-124-3p in human bladder cancer are elusive. Thus, this study was conducted to investigate the biological functions and its molecular mechanisms of miR-124-3p in human bladder cancer cell lines, discussing whether it has a potential to be a therapeutic biomarker of bladder cancer.MethodsThree human bladder cancer cell lines and samples from ten patients with bladder cancer were analyzed for the expression of miR-124-3p by quantitative RT--PCR. Exogenetic overexpression of miR-124-3p was established by transfecting mimics into T24, UM-UC-3 and J82 cells, after that cell proliferation and cell cycle were assessed by MTT assay, flow cytometry and Colony-forming assay. Cell motility and invasion ability were evaluated by wound healing assay and transwell assay. Tissue microarray, and immunohistochemistry with antibodies against ROCK1, MMP2 and MMP9 was performed using the peroxidase and DAB methods. The target gene of miR-124-3p was determined by luciferase assays, quantitative RT--PCR and western blot. The regulation of epithelial-to-mesenchymal transition by miR-124-3p was analyzed by western blot.ResultsmiR-124-3p is frequently down-regulated in bladder cancer both in three bladder cancer cell lines, T24, UM-UC-3, J82 and clinical samples. Overexpression of miR-124-3p induced G1-phase arrest in T24, UM-UC-3 and J82 cell lines and suppressed cell growth in colony-forming assay. miR-124-3p significantly repressed the capability of migration and invasion of bladder cancer cells. In addition, ROCK1 was identified as a new target of miR-124-3p. ROCK1, MMP2, MMP9 were up-regulated in bladder cancer tissues. Furthermore, we demonstrated miR-124-3p could inhibit bladder cancer cell epithelial mesenchymal transfer, and regulated the expression of c-Met, MMP2, MMP9.ConclusionsmiR-124-3p can repress the migration and invasion of bladder cancer cells via regulating ROCK1. Our data indicate that miR-124-3p could be a tumor suppressor and may have a potential to be a diagnostics or predictive biomarker in bladder cancer.

Dataset Information

MicroRNA?92a?3p inhibits the cell proliferation, migration and invasion of Wilms tumor by targeting NOTCH1.

Publications

MicroRNA‑92a‑3p inhibits the cell proliferation, migration and invasion of Wilms tumor by targeting NOTCH1.

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