Dataset Information

MicroRNA-103a inhibits gastric cancer cell proliferation, migration and invasion by targeting c-Myb.

ABSTRACT:

Objectives

There have been no previous reports concerning functions of miR-103a in gastric cancer (GC) cells. Thus the aim of the study was to investigate its expression and role in development of this tumour.

Materials and methods

Real-time RT-PCR was performed to detect expression of miR-103a in GC cell lines and clinical cancer specimens. To further understand its role, we restored expression of miR-103a in MGC-803 cell line by transfection with miR-103a mimics or inhibitors. Effects of miR-103a on cell proliferation, migration and invasion on targets were also determined.

Results

miR-103a was down-regulated in both GC cell lines and clinical cancer specimens. Meanwhile, its level was closely associated with pM or pTNM stage of GC. Overexpression of miR-103a markedly suppressed proliferation, migration, and invasion of GC cells, while its inhibition significantly accelerated cell proliferation, migration and invasion. Moreover, c-Myb was identified to be a functional downstream target of miR-103a, ectopic expression of which partially reversed suppression of cell proliferation and invasion.

Conclusions

Thus our observations suggest that miR-103a functioned as a tumour suppressor by targeting c-Myb. These findings indicate that miR-103a might play a significant role in pathogenesis of GC.

SUBMITTER: Liang J

PROVIDER: S-EPMC6496034 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Publications

MicroRNA-103a inhibits gastric cancer cell proliferation, migration and invasion by targeting c-Myb.

Liang Jinlong J Liu Xianfeng X Xue Hongpeng H Qiu Bing B Wei Bin B Sun Kan K

Cell proliferation 20141222 1

<h4>Objectives</h4>There have been no previous reports concerning functions of miR-103a in gastric cancer (GC) cells. Thus the aim of the study was to investigate its expression and role in development of this tumour.<h4>Materials and methods</h4>Real-time RT-PCR was performed to detect expression of miR-103a in GC cell lines and clinical cancer specimens. To further understand its role, we restored expression of miR-103a in MGC-803 cell line by transfection with miR-103a mimics or inhibitors. E ...[more]

PMID: 25530421

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Dataset Information

MicroRNA-103a inhibits gastric cancer cell proliferation, migration and invasion by targeting c-Myb.

Objectives

Materials and methods

Results

Conclusions

Publications

MicroRNA-103a inhibits gastric cancer cell proliferation, migration and invasion by targeting c-Myb.

Similar Datasets

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