Ontology highlight
ABSTRACT:
SUBMITTER: Kim S
PROVIDER: S-EPMC6072530 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Kim Sunghyun S Sung Daekyung D Chang Jeong Ho JH
MedChemComm 20171102 1
In this study, we prepared protein A grafted magnetic nanoparticles for the industrial large-scale purification of antibodies with enhancement of binding capacity and immobilization by controlled orientation with chlorophenylsilane (CPTMS) on the surface. For site-specific immobilization of protein A, genetically modified protein A with a cysteine residue was expressed in <i>E. coli</i> and purified by affinity chromatography. To improve the surface area to volume ratio and increase the immobili ...[more]