Ontology highlight
ABSTRACT:
SUBMITTER: Mahajan R
PROVIDER: S-EPMC6471088 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Mahajan Rashmi R Rouhi Mona M Shinde Sudhirkumar S Bedwell Thomas T Incel Anil A Mavliutova Liliia L Piletsky Sergey S Nicholls Ian A IA Sellergren Börje B
Angewandte Chemie (International ed. in English) 20181115 3
We report an approach integrating the synthesis of protein-imprinted nanogels ("plastic antibodies") with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised b ...[more]