Dataset Information

Microbiota of Palm Oil Mill Wastewater in Malaysia.

ABSTRACT: This study was aimed at identifying indigenous microorganisms from palm oil mill effluent (POME) and to ascertain the microbial load. Isolation and identification of indigenous microorganisms was subjected to standard microbiological methods and sequencing of the 16S rRNA and 18S rRNA genes. Sequencing of the 16S rRNA and 18S rRNA genes for the microbial strains signifies that they were known as Micrococcus luteus 101PB, Stenotrophomonas maltophilia 102PB, Bacillus cereus 103PB, Providencia vermicola 104PB, Klebsiella pneumoniae 105PB, Bacillus subtilis 106PB, Aspergillus fumigatus 107PF, Aspergillus nomius 108PF, Aspergillus niger 109PF and Meyerozyma guilliermondii 110PF. Results revealed that the population of total heterotrophic bacteria (THB) ranged from 9.5 × 105 - 7.9 × 106 cfu/mL. The total heterotrophic fungi (THF) ranged from 2.1 × 104 - 6.4 × 104 cfu/mL. Total viable heterotrophic indigenous microbial population on CMC agar ranged from 8.2 × 105 - 9.1 × 106 cfu/mL and 1.4 × 103 - 3.4 × 103 cfu/mL for bacteria and fungi respectively. The microbial population of oil degrading bacteria (ODB) ranged from 6.4 × 105 - 4.8 × 106 cfu/mL and the oil degrading fungi (ODF) ranged from 2.8 × 103 - 4.7 × 104 cfu/mL. The findings revealed that microorganisms flourish well in POME. Therefore, this denotes that isolating native microorganisms from POME is imperative for effectual bioremediation, biotreatment and biodegradation of industrial wastewaters.

SUBMITTER: Bala JD

PROVIDER: S-EPMC6072722 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Microbiota of Palm Oil Mill Wastewater in Malaysia.

Bala Jeremiah David JD Lalung Japareng J Al-Gheethi Adel Ali Saeed AAS Hossain Kaizar K Ismail Norli N

Tropical life sciences research 20180706 2

This study was aimed at identifying indigenous microorganisms from palm oil mill effluent (POME) and to ascertain the microbial load. Isolation and identification of indigenous microorganisms was subjected to standard microbiological methods and sequencing of the 16S rRNA and 18S rRNA genes. Sequencing of the 16S rRNA and 18S rRNA genes for the microbial strains signifies that they were known as Micrococcus luteus 101PB, Stenotrophomonas maltophilia 102PB, Bacillus cereus 10 ...[more]

PMID: 30112146

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Project description:BackgroundThermophilic Bacillus strains of phylogenetic Bacillus rRNA group 5 were described as a new genus Geobacillus. Their geographical distribution included oilfields, hay compost, hydrothermal vent or soils. The members from the genus Geobacillus have a growth temperatures ranging from 35 to 78 degrees C and contained iso-branched saturated fatty acids (iso-15:0, iso-16:0 and iso-17:0) as the major fatty acids. The members of Geobacillus have similarity in their 16S rRNA gene sequences (96.5-99.2%). Thermophiles harboring intrinsically stable enzymes are suitable for industrial applications. The quest for intrinsically thermostable lipases from thermophiles is a prominent task due to the laborious processes via genetic modification.ResultsTwenty-nine putative lipase producers were screened and isolated from palm oil mill effluent in Malaysia. Of these, isolate T1T was chosen for further study as relatively higher lipase activity was detected quantitatively. The crude T1 lipase showed high optimum temperature of 70 degrees C and was also stable up to 60 degrees C without significant loss of crude enzyme activity. Strain T1T was a Gram-positive, rod-shaped, endospore forming bacterium. On the basic of 16S rDNA analysis, strain T1T was shown to belong to the Bacillus rRNA group 5 related to Geobacillus thermoleovorans (DSM 5366T) and Geobacillus kaustophilus (DSM 7263T). Chemotaxonomic data of cellular fatty acids supported the affiliation of strain T1T to the genus Geobacillus. The results of physiological and biochemical tests, DNA/DNA hybridization, RiboPrint analysis, the length of lipase gene and protein pattern allowed genotypic and phenotypic differentiation of strain T1T from its validly published closest phylogenetic neighbors. Strain T1T therefore represents a novel species, for which the name Geobacillus zalihae sp. nov. is proposed, with the type strain T1T (=DSM 18318T; NBRC 101842T).ConclusionStrain T1T was able to secrete extracellular thermostable lipase into culture medium. The strain T1T was identified as Geobacillus zalihae T1T as it differs from its type strains Geobacillus kaustophilus (DSM 7263T) and Geobacillus thermoleovorans (DSM 5366T) on some physiological studies, cellular fatty acids composition, RiboPrint analysis, length of lipase gene and protein profile.

Project description:Agricultural expansion is one of the leading causes of deforestation in the tropics and in Southeast Asia it is predominantly driven by large-scale production for international trade. Peninsular Malaysia has a long history of plantation agriculture and has been a predominantly resource-based economy where expanding plantations like those of oil palm continue to replace natural forests. Habitat loss from deforestation and expanding plantations threatens Malaysian biodiversity. Expanding industrial plantations have also been responsible for drainage and conversions of peatland forests resulting in release of large amounts of carbon dioxide. The demand for palm oil is expected to increase further and result in greater pressures on tropical forests. Given Malaysia's high biophysical suitability for oil palm cultivation, it is important to understand patterns of oil palm expansion to better predict forest areas that are vulnerable to future expansion. We study natural forest conversion to industrial oil palm in Peninsular Malaysia between 1988 and 2012 to identify determinants of recent oil palm expansion using logistic regression and hierarchical partitioning. Using maps of recent conversions and remaining forests, we characterize agro-environmental suitability and accessibility for the past and future conversions. We find that accessibility to previously existing plantations is the strongest determinant of oil palm expansion and is significant throughout the study period. Almost all (> 99%) of the forest loss between 1988 and 2012 that has been converted to industrial oil palm plantations is within 1 km from oil palm plantations that have been established earlier. Although most forest conversions to industrial oil palm have been in areas of high biophysical suitability, there has been an increase in converted area in regions with low oil palm suitability since 2006. We find that reduced suitability does not necessarily restrict conversions to industrial oil palm in the region; however, lack of access to established plantations does.

Dataset Information

Microbiota of Palm Oil Mill Wastewater in Malaysia.

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Microbiota of Palm Oil Mill Wastewater in Malaysia.

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