Dataset Information

Investigation of Three-Dimensional Microstructure of Tricalcium Silicate (C?S) by Electron Microscopy.

ABSTRACT: A serial block-face scanning electron microscopy (SBFSEM) system, composed of a scanning electron microscope (SEM) and an ultra-microtome installed within the SEM vacuum chamber, has been used to characterize the three-dimensional (3D) microstructure of tricalcium silicate (C?S) grains embedded in epoxy resin. A selection of C?S grains were segmented and rendered with 3D-image processing software, which allowed the C?S grains to be clearly visualized and enabled statistically quantitative analysis. The results show that about 5% of the C?S grains have volumes larger than 1 μm³ and the average volume of the grains is 25 μm³. Pores can also be clearly seen in the biggest C?S grain, the volume of which is 3.6 × 10? μm³, and the mean volume and total volume of all the pores within this grain are 4.8 μm³ and 3.0 × 10³ μm³, respectively. The reported work provides a new approach for the characterization of the 3D spatial structure of raw C?S materials, and the resulting 3D structure of the raw C?S is important for further systematic research on the relationships between the spatial microstructure and the hydration kinetics of C?S and other cement minerals.

SUBMITTER: Yang F

PROVIDER: S-EPMC6073500 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Investigation of Three-Dimensional Microstructure of Tricalcium Silicate (C₃S) by Electron Microscopy.

Yang Fei F Liu Xianping X Zhao Yongjuan Y Zhang Yongming Y Wang Peiming P Robinson Ian I Chen Bo B

Materials (Basel, Switzerland) 20180629 7

A serial block-face scanning electron microscopy (SBFSEM) system, composed of a scanning electron microscope (SEM) and an ultra-microtome installed within the SEM vacuum chamber, has been used to characterize the three-dimensional (3D) microstructure of tricalcium silicate (C₃S) grains embedded in epoxy resin. A selection of C₃S grains were segmented and rendered with 3D-image processing software, which allowed the C₃S grains to be clearly visualized and enabled statistically quantitative analys ...[more]

PMID: 29966230

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Project description:Unlike white and brown adipose tissues, the bone marrow adipocyte (BMA) exists in a microenvironment containing unique populations of hematopoietic and skeletal cells. To study this microenvironment at the sub-cellular level, we performed a three-dimensional analysis of the ultrastructure of the BMA niche with focused ion beam scanning electron microscopy (FIB-SEM). This revealed that BMAs display hallmarks of metabolically active cells including polarized lipid deposits, a dense mitochondrial network, and areas of endoplasmic reticulum. The distinct orientations of the triacylglycerol droplets suggest that fatty acids are taken up and/or released in three key areas - at the endothelial interface, into the hematopoietic milieu, and at the bone surface. Near the sinusoidal vasculature, endothelial cells send finger-like projections into the surface of the BMA which terminate near regions of lipid within the BMA cytoplasm. In some regions, perivascular cells encase the BMA with their flattened cellular projections, limiting contacts with other cells in the niche. In the hematopoietic milieu, BMAT adipocytes of the proximal tibia interact extensively with maturing cells of the myeloid/granulocyte lineage. Associations with erythroblast islands are also prominent. At the bone surface, the BMA extends organelle and lipid-rich cytoplasmic regions toward areas of active osteoblasts. This suggests that the BMA may serve to partition nutrient utilization between diverse cellular compartments, serving as an energy-rich hub of the stromal-reticular network. Lastly, though immuno-EM, we've identified a subset of bone marrow adipocytes that are innervated by the sympathetic nervous system, providing an additional mechanism for regulation of the BMA. In summary, this work reveals that the bone marrow adipocyte is a dynamic cell with substantial capacity for interactions with the diverse components of its surrounding microenvironment. These local interactions likely contribute to its unique regulation relative to peripheral adipose tissues.

Dataset Information

Investigation of Three-Dimensional Microstructure of Tricalcium Silicate (C?S) by Electron Microscopy.

Publications

Investigation of Three-Dimensional Microstructure of Tricalcium Silicate (C₃S) by Electron Microscopy.

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