Ontology highlight
ABSTRACT:
SUBMITTER: Adiconis X
PROVIDER: S-EPMC6075671 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Nature methods 20180604 7
Specialized RNA-seq methods are required to identify the 5' ends of transcripts, which are critical for studies of gene regulation, but these methods have not been systematically benchmarked. We directly compared six such methods, including the performance of five methods on a single human cellular RNA sample and a new spike-in RNA assay that helps circumvent challenges resulting from uncertainties in annotation and RNA processing. We found that the 'cap analysis of gene expression' (CAGE) metho ...[more]