The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients.
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ABSTRACT:
SUBMITTER: Huang Z
PROVIDER: S-EPMC6075744 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients.
Huang Zhu Z Du Chi-Xin CX Pan Xiao-Dong XD
PloS one 20180803 8
Tear is an accessible fluid for exploring biomarkers of dry eye disease. This study describes a fast proteomic method by LC-Q-orbitrap-MS analysis with in-strip digestion and investigates the tear proteome of dry eye patients. Schirmer's strips were used for collection of tear fluid from patients. These strips were cut into pieces and directly digested with trypsin before mass spectrometry analysis. The data showed that more than 50 proteins were found in tear fluid from dry eye patients. Gene O ...[more]
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