Dataset Information

Genome-wide SWAp-Tag yeast libraries for proteome exploration.

ABSTRACT: Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

SUBMITTER: Weill U

PROVIDER: S-EPMC6076999 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Genome-wide SWAp-Tag yeast libraries for proteome exploration.

Weill Uri U Yofe Ido I Sass Ehud E Stynen Bram B Davidi Dan D Natarajan Janani J Ben-Menachem Reut R Avihou Zohar Z Goldman Omer O Harpaz Nofar N Chuartzman Silvia S Kniazev Kiril K Knoblach Barbara B Laborenz Janina J Boos Felix F Kowarzyk Jacqueline J Ben-Dor Shifra S Zalckvar Einat E Herrmann Johannes M JM Rachubinski Richard A RA Pines Ophry O Rapaport Doron D Michnick Stephen W SW Levy Emmanuel D ED Schuldiner Maya M

Nature methods 20180709 8

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragment ...[more]

PMID: 29988094

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Dataset Information

Genome-wide SWAp-Tag yeast libraries for proteome exploration.

Publications

Genome-wide SWAp-Tag yeast libraries for proteome exploration.

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