Genome-wide SWAp-Tag yeast libraries for proteome exploration.
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ABSTRACT:
SUBMITTER: Weill U
PROVIDER: S-EPMC6076999 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
Genome-wide SWAp-Tag yeast libraries for proteome exploration.
Weill Uri U Yofe Ido I Sass Ehud E Stynen Bram B Davidi Dan D Natarajan Janani J Ben-Menachem Reut R Avihou Zohar Z Goldman Omer O Harpaz Nofar N Chuartzman Silvia S Kniazev Kiril K Knoblach Barbara B Laborenz Janina J Boos Felix F Kowarzyk Jacqueline J Ben-Dor Shifra S Zalckvar Einat E Herrmann Johannes M JM Rachubinski Richard A RA Pines Ophry O Rapaport Doron D Michnick Stephen W SW Levy Emmanuel D ED Schuldiner Maya M
Nature methods 20180709 8
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragment ...[more]
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