Transcriptomic signatures reveal immune dysregulation in human diabetic and idiopathic gastroparesis.

ABSTRACT: BACKGROUND:Cellular changes described in human gastroparesis have revealed a role for immune dysregulation, however, a mechanistic understanding of human gastroparesis and the signaling pathways involved are still unclear. METHODS:Diabetic gastroparetics, diabetic non-gastroparetic controls, idiopathic gastroparetics and non-diabetic non-gastroparetic controls underwent full-thickness gastric body biopsies. Deep RNA sequencing was performed and pathway analysis of differentially expressed transcripts was done using Ingenuity®. A subset of differentially expressed genes in diabetic gastroparesis was validated in a separate cohort using QT-PCR. RESULTS:111 genes were differentially expressed in diabetic gastroparesis and 181 in idiopathic gastroparesis with a log2fold difference of |???2| and false detection rate (FDR)

SUBMITTER: Grover M 

PROVIDER: S-EPMC6081936 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature