Dataset Information

Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of ?-cryptoxanthin by Kocuria marina DAGII.

ABSTRACT: The dairy industry produces enormous amount of cheese whey containing the major milk nutrients, but this remains unutilized all over the globe. The present study investigates the production of ?-cryptoxanthin (?-CRX) by Kocuria marina DAGII using cheese whey as substrate. Response surface methodology (RSM) and an artificial neural network (ANN) approach were implemented to obtain the maximum ?-CRX yield. Significant factors, i.e. yeast extract, peptone, cheese whey and initial pH, were the input variables in both the optimizing studies, and ?-CRX yield and biomass were taken as output variables. The ANN topology of 4-9-2 was found to be optimum when trained with a feed-forward back-propagation algorithm. Experimental values of ?-CRX yield (17.14?mg?l-1) and biomass (5.35?g?l-1) were compared and ANN predicted values (16.99?mg?l-1 and 5.33?g?l-1, respectively) were found to be more accurate compared with RSM predicted values (16.95?mg?l-1 and 5.23?g?l-1, respectively). Detailed kinetic analysis of cellular growth, substrate consumption and product formation revealed that growth inhibition took place at substrate concentrations higher than 12% (v/v) of cheese whey. The Han and Levenspiel model was the best fitted substrate inhibition model that described the cell growth in cheese whey with an R2 and MSE of 0.9982% and 0.00477%, respectively. The potential importance of this study lies in the development, optimization and modelling of a suitable cheese whey supplemented medium for increased ?-CRX production.

SUBMITTER: Mitra R

PROVIDER: S-EPMC6083662 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of β-cryptoxanthin by <i>Kocuria marina</i> DAGII.

Mitra Ruchira R Dutta Debjani D

Royal Society open science 20180711 7

The dairy industry produces enormous amount of cheese whey containing the major milk nutrients, but this remains unutilized all over the globe. The present study investigates the production of β-cryptoxanthin (β-CRX) by <i>Kocuria marina</i> DAGII using cheese whey as substrate. Response surface methodology (RSM) and an artificial neural network (ANN) approach were implemented to obtain the maximum β-CRX yield. Significant factors, i.e. yeast extract, peptone, cheese whey and initial pH, were th ...[more]

PMID: 30109058

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Project description:BackgroundDelactosed whey permeate (DWP) is a side stream of whey processing, which often is discarded as waste, despite of its high residual content of lactose, typically 10-20%. Microbial fermentation is one of the most promising approaches for valorizing nutrient rich industrial waste streams, including those generated by the dairies. Here we present a novel microbial platform specifically designed to generate useful compounds from dairy waste. As a starting point we use Corynebacterium glutamicum, an important workhorse used for production of amino acids and other important compounds, which we have rewired and complemented with genes needed for lactose utilization. To demonstrate the potential of this novel platform we produce ethanol from lactose in DWP.ResultsFirst, we introduced the lacSZ operon from Streptococcus thermophilus, encoding a lactose transporter and a β-galactosidase, and achieved slow growth on lactose. The strain could metabolize the glucose moiety of lactose, and galactose accumulated in the medium. After complementing with the Leloir pathway (galMKTE) from Lactococcus lactis, co-metabolization of galactose and glucose was accomplished. To further improve the growth and increase the sugar utilization rate, the strain underwent adaptive evolution in lactose minimal medium for 100 generations. The outcome was strain JS95 that grew fast in lactose mineral medium. Nevertheless, JS95 still grew poorly in DWP. The growth and final biomass accumulation were greatly stimulated after supplementation with NH4+, Mn2+, Fe2+ and trace minerals. In only 24 h of cultivation, a high cell density (OD600 of 56.8 ± 1.3) was attained. To demonstrate the usefulness of the platform, we introduced a plasmid expressing pyruvate decarboxylase and alcohol dehydrogenase, and managed to channel the metabolic flux towards ethanol. Under oxygen-deprived conditions, non-growing suspended cells could convert 100 g/L lactose into 46.1 ± 1.4 g/L ethanol in DWP, a yield of 88% of the theoretical. The resting cells could be re-used at least three times, and the ethanol productivities obtained were 0.96 g/L/h, 2.2 g/L/h, and 1.6 g/L/h, respectively.ConclusionsAn efficient process for producing ethanol from DWP, based on C. glutamicum, was demonstrated. The results obtained clearly show a great potential for this newly developed platform for producing value-added chemicals from dairy waste.

Dataset Information

Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of ?-cryptoxanthin by Kocuria marina DAGII.

Publications

Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of β-cryptoxanthin by <i>Kocuria marina</i> DAGII.

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