Dataset Information

Alteration of HDL Protein Composition with Hemodialysis Initiation.

ABSTRACT:

Background and objectives

HDL particles obtained from patients on chronic hemodialysis exhibit lower cholesterol efflux capacity and are enriched in inflammatory proteins compared with those in healthy individuals. Observed alterations in HDL proteins could be due to effects of CKD, but also may be influenced by the hemodialysis procedure, which stimulates proinflammatory and prothrombotic pathways.

Design, setting, participants, & measurements

We compared HDL-associated proteins in 143 participants who initiated hemodialysis within the previous year with those of 110 participants with advanced CKD from the Hemodialysis Fistula Maturation Study. We quantified concentrations of 38 HDL-associated proteins relative to total HDL protein using targeted mass spectrometry assays that included a stable isotope-labeled internal standard. We used linear regression to compare the relative abundances of HDL-associated proteins after adjustment and required a false discovery rate q value ?10% to control for multiple testing. We further assessed the association between hemodialysis initiation and cholesterol efflux capacity in a subset of 80 participants.

Results

After adjustment for demographics, comorbidities, and other clinical characteristics, eight HDL-associated proteins met the prespecified false discovery threshold for association. Recent hemodialysis initiation was associated with higher HDL-associated concentrations of serum amyloid A1, A2, and A4; hemoglobin-?; haptoglobin-related protein; cholesterylester transfer protein; phospholipid transfer protein; and apo E. The trend for participants recently initiating hemodialysis for lower cholesterol efflux capacity compared with individuals with advanced CKD did not reach statistical significance.

Conclusions

Compared with advanced CKD, hemodialysis initiation within the previous year is associated with higher concentrations of eight HDL proteins related to inflammation and lipid metabolism. Identified associations differ from those recently observed for nondialysis-requiring CKD. Hemodialysis initiation may further impair cholesterol efflux capacity. Further work is needed to clarify the clinical significance of the identified proteins with respect to cardiovascular risk.

Podcast

This article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2018_07_25_CJASNPodcast_18_8_W.mp3.

SUBMITTER: Wang K

PROVIDER: S-EPMC6086713 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Publications

Alteration of HDL Protein Composition with Hemodialysis Initiation.

Wang Ke K Zelnick Leila R LR Hoofnagle Andrew N AN Vaisar Tomas T Henderson Clark M CM Imrey Peter B PB Robinson-Cohen Cassianne C de Boer Ian H IH Shiu Yan-Ting YT Himmelfarb Jonathan J Beck Gerald J GJ Kestenbaum Bryan B

Clinical journal of the American Society of Nephrology : CJASN 20180725 8

<h4>Background and objectives</h4>HDL particles obtained from patients on chronic hemodialysis exhibit lower cholesterol efflux capacity and are enriched in inflammatory proteins compared with those in healthy individuals. Observed alterations in HDL proteins could be due to effects of CKD, but also may be influenced by the hemodialysis procedure, which stimulates proinflammatory and prothrombotic pathways.<h4>Design, setting, participants, & measurements</h4>We compared HDL-associated proteins ...[more]

PMID: 30045914

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Dataset Information

Alteration of HDL Protein Composition with Hemodialysis Initiation.

Background and objectives

Design, setting, participants, & measurements

Results

Conclusions

Podcast

Publications

Alteration of HDL Protein Composition with Hemodialysis Initiation.

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