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Highly efficient capture of cancer cells expressing EGFR by microfluidic methods based on antigen-antibody association.

ABSTRACT: Epidermal growth factor receptor (EGFR) was evaluated as a target antigen for cancer cell capture by microfluidic methods based on antigen-antibody association. A polymer CTC-chip microfluidic device was surface-functionalized with three different anti-EGFR antibodies and used to capture EGFR-expressing cancer cells. Capture efficacy depended on the type of antibody used, and cetuximab efficiently captured cancer cell lines that had a wide range of EGFR expression. Capture efficiency was analyzed from the viewpoint of antigen-antibody association in a kinetic process, i.e., cell rolling well-known in leukocyte adhesion, and antibodies with a smaller dissociation constant were shown to result in more efficient capture. Moreover, a lower limit of cellular EGFR expression level for the capture was estimated and methods to decrease the limit were discussed based on densities of anti-EGFR antibody on the device surface.

SUBMITTER: Ohnaga T

PROVIDER: S-EPMC6089922 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Highly efficient capture of cancer cells expressing EGFR by microfluidic methods based on antigen-antibody association.

Ohnaga Takashi T Takei Yoshinori Y Nagata Takuya T Shimada Yutaka Y

Scientific reports 20180813 1

Epidermal growth factor receptor (EGFR) was evaluated as a target antigen for cancer cell capture by microfluidic methods based on antigen-antibody association. A polymer CTC-chip microfluidic device was surface-functionalized with three different anti-EGFR antibodies and used to capture EGFR-expressing cancer cells. Capture efficacy depended on the type of antibody used, and cetuximab efficiently captured cancer cell lines that had a wide range of EGFR expression. Capture efficiency was analyze ...[more]

PMID: 30104638

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