Dataset Information

Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress.

ABSTRACT: The cis-acting element DRE/CRT plays an important role in activating gene expression responsive to osmotic stress, low temperature and high-salinity. DREB1/CBF genes encode DRE-binding proteins with the function as transcript activators. TaDREB1 was also found to be induced by osmotic stress.The dates of osmotic stress was assessed by seed germination drought resistance index; the full-length cDNA sequences of TaDREB1 gene were downloaded from NCBI datebase; identification of allelic variation and transcript expression were assessed by PCR and semi-quantitive RT-PCR analysis, respectively.Total 13 new allele variations of TaDREB1 were identified in the germplasms tested in the paper, including 5 TaDREB1-A on chromosome 3AL, 4 TaDREB1-B on chromosome 3BL and 4 TaDREB1-D on chromosome 3DL. In each variety, there existed two loci of TaDREB1-D genes, named TaDREB1-D1 and TaDREB1-D2, both of which had the similar nucleotide sequence except an 11 bp insertion in the former. In wheat seeds under osmotic stress, we did not detect the transcript expression level of TaDREB1-A and TaDREB1-B, but that of TaDREB1-D.The capacity of osmotic stress tolerance was closely correlated with the expression level and tendency of TaDREB1-D.

SUBMITTER: Liu M

PROVIDER: S-EPMC6090928 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Characterization of <i>TaDREB1</i> in wheat genotypes with different seed germination under osmotic stress.

Liu Meng M Wang Zeng Z Xiao Hong-Mei HM Yang Yan Y

Hereditas 20180801

<h4>Background</h4>The cis-acting element DRE/CRT plays an important role in activating gene expression responsive to osmotic stress, low temperature and high-salinity. <i>DREB1</i>/<i>CBF</i> genes encode DRE-binding proteins with the function as transcript activators. <i>TaDREB1</i> was also found to be induced by osmotic stress.<h4>Methods</h4>The dates of osmotic stress was assessed by seed germination drought resistance index; the full-length cDNA sequences of <i>TaDREB1</i> gene were downl ...[more]

PMID: 30123102

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Project description:Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10-100 μM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H2O2), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na+, Cl-). Meanwhile, the contents of melatonin, soluble proteins, and K+ as well as the K+/Na+ balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H2O2, MDA, Na+, and Cl-. The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K+/Na+ contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 μM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance.

Project description:Soybean (Glycine max (L.) Merr.) is important to the global food industry; however, its productivity is affected by abiotic stresses such as osmosis, flooding, heat, and cold. Here, we evaluated the bioactive extracts of two biostimulant bacterial strains, Bacillus butanolivorans KJ40 and B. siamensis H30-3, for their ability to convey tolerance to osmotic stress in soybean seeds during germination. Soybean seeds were dip-treated in extracts of KJ40 (KJ40E) or H30-3 (H30-3E) and incubated with either 0% or 20% polyethylene glycol 6000 (PEG), simulating drought-induced osmotic stress. We measured malondialdehyde content as a marker for lipid peroxidation, as well as the activity of antioxidant enzymes, including catalase, glutathione peroxidase, and glutathione reductase, together with changes in sugars content. We also monitored the expression of genes involved in the gibberellic acid (GA)-biosynthesis pathway, and abscisic acid (ABA) signaling. Following osmotic stress in the extract-treated seeds, malondialdehyde content decreased, while antioxidant enzyme activity increased. Similarly, the expression of GA-synthesis genes, including GmGA2ox1 and GmGA3 were upregulated in KJ40E-dipped seeds at 12 or 6 h after treatment, respectively. The ABA signaling genes GmABI4 and GmDREB1 were upregulated in H30-3E- and KJ40E-treated seeds at 0 and 12 h after treatment under osmotic stress; however, GmABI5, GmABI4, and GmDREB1 levels were also elevated in the dip-treated seeds in baseline conditions. The GA/ABA ratio increased only in KJ40E-treated seeds undergoing osmotic stress, while glucose content significantly decreased in H30-3E-treated seeds at 24 h after treatment. Collectively, our findings indicated that dip-treatment of soybean seeds in KJ40E and H30-3E can enhance the seeds' resistance to osmotic stress during germination, and ameliorate cellular damage caused by secondary oxidative stress. This seed treatment can be used agriculturally to promote germination under drought stress and lead to increase crop yield and quality.

Dataset Information

Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress.

Publications

Characterization of <i>TaDREB1</i> in wheat genotypes with different seed germination under osmotic stress.

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