Cellular reagents for diagnostics and synthetic biology.
Ontology highlight
ABSTRACT:
SUBMITTER: Bhadra S
PROVIDER: S-EPMC6093680 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Cellular reagents for diagnostics and synthetic biology.
Bhadra Sanchita S Pothukuchy Arti A Shroff Raghav R Cole Austin W AW Byrom Michelle M Ellefson Jared W JW Gollihar Jimmy D JD Ellington Andrew D AD
PloS one 20180815 8
We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a variety of molecular diagnostics, such as TaqMan qPCR with no diminution in sensitivity, and in synthetic biology cornerstones such as the Gibson assembly of DNA fragments, where new plasmids can be constructed solely based on adding cellular reagents ...[more]
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