Dataset Information

Association between AKT rs2494752 single nucleotide polymorphism and the risk of metastasis in hepatocellular carcinoma.

ABSTRACT: Hepatocellular carcinoma (HCC) is one of the most common types of human tumors, which is characterized by high morbidity and mortality rates. AKT1 transcriptional activity is implicated in HCC initiation and development. In the present study, the effects of rs2494752 single nucleotide polymorphism (SNP) on AKT1 transcriptional activity in the progression of HCC cells were investigated. A case-control study was analyzed in 1,056 HCC patients and 1,080 healthy individuals using the PCR assay method. Results indicated AKT1 expression levels were up-regulated in HCC tissue compared to adjacent normal tissues. Furthermore, a higher frequency of AKT rs2494752 AG and AA genotypes were observed in HCC cases (P=0.0046). Gene polymorphism identified C and T alleles were frequency in HCC patients compared to healthy individuals. Individuals harboring AKT rs2494752 AG/AA genotype had a vital increased susceptibility to HCC in the dominant model (P=0.0028). In addition, AKT1 rs2494752 GG genotype showed an increasing of AKT1 promoter activity determined by the luciferase assay. Furthermore, it was demonstrated that AKT1 rs2494752 GG and C polymorphism was more aggressive than other AKT1 rs2494752 cancer cells. Moreover, AKT1 rs2494752 GG markedly increased rates of response to NCT chemotherapy. Additionally, results revealed that AKT1 rs2494752 GG and C increased the risk factors of HCC. In conclusion, these results indicate that AKT1 rs2494752 polymorphisms may be regarded as a candidate gene in assessing the susceptibility, metastasis and responses to chemotherapy in the progression of hepatocellular carcinoma.

SUBMITTER: Wang Z

PROVIDER: S-EPMC6096108 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Association between <i>AKT</i> rs2494752 single nucleotide polymorphism and the risk of metastasis in hepatocellular carcinoma.

Wang Zhaohui Z Fu Huiling H Li Wei W

Oncology letters 20180702 3

Hepatocellular carcinoma (HCC) is one of the most common types of human tumors, which is characterized by high morbidity and mortality rates. AKT1 transcriptional activity is implicated in HCC initiation and development. In the present study, the effects of rs2494752 single nucleotide polymorphism (SNP) on AKT1 transcriptional activity in the progression of HCC cells were investigated. A case-control study was analyzed in 1,056 HCC patients and 1,080 healthy individuals using the PCR assay metho ...[more]

PMID: 30127980

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Project description:BackgroundCyclin D1 (CCND1) G870A polymorphism may be associated with hepatocellular carcinoma (HCC) risk, but the results of previous studies were inconsistent. Available evidence was meta-analyzed to assess their potential association.MethodsDatabases PubMed, EMBASE, Cochrane Library, Web of Science, Chinese Biomedical Literature database, China National Knowledge Infrastructure, and Google Scholar were systematically searched. Meta-analyses were performed to investigate the association of G870A polymorphism with HCC risk by calculating odds ratios (ORs) and 95% confidence intervals (CIs) from the data of relevant case-control studies.ResultsResults of this meta-analysis of six case-control studies involving 1,030 cases and 1,683 controls indicate that G870A polymorphism was not associated with HCC risk in any of the five genetic models tested (recessive model: AA vs GG + AG: OR =1.38, 95% CI =0.95-2.00, P=0.09; dominant model: AG + AA vs GG: OR =1.38, 95% CI =0.87-2.20, P=0.17; homozygous model: AA vs GG: OR =1.60, 95% CI =0.87-2.94, P=0.13; heterozygous model: AG vs GG: OR =1.24, 95% CI =0.86-1.79, P=0.25; allelic model: A vs G: OR =1.30, 95% CI =0.95-1.80, P=0.10). Subgroup analyses according to ethnicity showing marginally significant association between this single nucleotide polymorphism and HCC risk indicate that G870A may be significantly associated with HCC risk in Caucasian populations (recessive model: AA vs GG + AG: OR =2.34, 95% CI =1.60-3.42, P<0.0001; dominant model: AG + AA vs GG: OR =2.44, 95% CI =1.19-4.97, P=0.01; homozygous model: AA vs GG: OR =3.42, 95% CI =1.80-6.50, P=0.0002; allelic model: A vs G: OR =2.06, 95% CI =1.31-3.24, P=0.002), but not in Asian populations.ConclusionAvailable evidence suggests that no significant association between G870A polymorphism and HCC risk was found in either total populations or Asian populations. However, significant association was found in Caucasian populations. These results should be verified and extended in further detailed and well-designed studies involving larger, multiethnic samples.

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Dataset Information

Association between AKT rs2494752 single nucleotide polymorphism and the risk of metastasis in hepatocellular carcinoma.

Publications

Association between <i>AKT</i> rs2494752 single nucleotide polymorphism and the risk of metastasis in hepatocellular carcinoma.

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