Unknown

Dataset Information

0

Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation.


ABSTRACT: Microglial cells as innate immune key players have a critical and unique role in neurodegenerative disorders. They strongly interact with their microenvironment in a complex manner and react to changes by switching their phenotype and functional activation states. In order to understand the development of brain diseases, it is imperative to elucidate up- or down-regulation of genes involved in microglia polarisation in time-profile by a simple-to-use strategy. Here, we present a new imaging strategy to follow promoter activity of genes involved in microglia polarisation. We lentivirally transduced BV-2 microglia cells in culture with constructs consisting of the induced nitric oxide synthase (iNOS), Fc gamma receptor III (Fcgr3) (both resembling the pro-inflammatory M1-like phenotype) or Chitinase-like 3 (Chil3/Ym1) (resembling the anti-inflammatory M2-like phenotype) promoters and stimulated transgenic cells with potent activators for pro- or anti-inflammatory response, such as lipopolysaccharide (LPS)?+?interferon gamma (IFN-?) or interleukin (IL)-4, respectively. Promoter activities upon polarisation phases were quantitatively assessed by the two imaging reporters Luc2 for bioluminescence and eGFP for fluorescence.

SUBMITTER: Collmann FM 

PROVIDER: S-EPMC6096558 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation.

Collmann Franziska M FM   Pijnenburg Rory R   Schneider Gabriele G   Schäfer Cordula C   Folz-Donahue Kat K   Kukat Christian C   Hoehn Mathias M  

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20180522 3


Microglial cells as innate immune key players have a critical and unique role in neurodegenerative disorders. They strongly interact with their microenvironment in a complex manner and react to changes by switching their phenotype and functional activation states. In order to understand the development of brain diseases, it is imperative to elucidate up- or down-regulation of genes involved in microglia polarisation in time-profile by a simple-to-use strategy. Here, we present a new imaging stra  ...[more]

Similar Datasets

| S-EPMC9614552 | biostudies-literature
| S-EPMC6469801 | biostudies-literature
| S-EPMC3294422 | biostudies-literature
2016-12-19 | GSE92588 | GEO
| S-EPMC7160884 | biostudies-literature
| S-EPMC6430126 | biostudies-literature
| S-EPMC3387985 | biostudies-literature
| S-EPMC4113417 | biostudies-literature
| S-EPMC9881286 | biostudies-literature
| S-EPMC3510088 | biostudies-literature