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Detection of microcystin-producing cyanobacteria in water samples using loop-mediated isothermal amplification targeting mcyB gene.

ABSTRACT: Microcystin toxin-producing cyanobacteria are known to have harmful effects on humans and animals. We have developed a loop-mediated isothermal amplification (LAMP)-based detection method by targeting the microcystin synthetase B gene (mcyB), the gene responsible for the production of microcystin. The sensitivity of the method was found to be 1 fg per reaction, and it was 1000-fold higher than the conventional PCR. The LAMP method was able to amplify the target gene with a minimum amount of dNTP (0.4 mM), which further reduces the cost of reaction. The improved LAMP assay could detect the presence of the toxin-producing cyanobacteria in water samples within 2 h of time, which demonstrates the rapidness of the method. Freshwater samples were screened using the developed LAMP, and seven water samples collected from lakes and a bird sanctuary tested positive for mcyB gene harboring cyanobacteria, and negative in all other drinking waters. Hence, the developed LAMP could be a possible alternative to the existing molecular methods for screening for microcystin in environmental samples with greater sensitivity.

SUBMITTER: Ramya M

PROVIDER: S-EPMC6097961 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.

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Development of a loop-mediated isothermal amplification assay for detection of <i>Austropeplea tomentosa</i> from environmental water samples.

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| S-EPMC6646635 | biostudies-literature

Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae.

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| S-EPMC2446398 | biostudies-literature

Detection of Shigella in Milk and Clinical Samples by Magnetic Immunocaptured-Loop-Mediated Isothermal Amplification Assay.

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| S-EPMC5807921 | biostudies-literature

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