Ontology highlight
ABSTRACT:
SUBMITTER: Cho HS
PROVIDER: S-EPMC7165947 | biostudies-literature | 2005 Nov
REPOSITORIES: biostudies-literature
Journal of veterinary medicine. B, Infectious diseases and veterinary public health 20051101 9
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was used to detect canine distemper virus (CDV) genomic RNA. A set of four primers, two outer and two inner, were designed from CDV genomic RNA targeting the nucleocapsid protein gene. The optimal reaction time and temperature for LAMP were determined to be 60 min at 65 degrees C. The relative sensitivity and specificity of RT-LAMP was found to be 100% and 93.3%, respectively, based on 50 canine blood samples and using RT-PCR ...[more]