Glibenclamide Produces Region-Dependent Effects on Cerebral Edema in a Combined Injury Model of Traumatic Brain Injury and Hemorrhagic Shock in Mice.

ABSTRACT: Cerebral edema is critical to morbidity/mortality in traumatic brain injury (TBI) and is worsened by hypotension. Glibenclamide may reduce cerebral edema by inhibiting sulfonylurea receptor-1 (Sur1); its effect on diffuse cerebral edema exacerbated by hypotension/resuscitation is unknown. We aimed to determine if glibenclamide improves pericontusional and/or diffuse edema in controlled cortical impact (CCI) (5m/sec, 1?mm depth) plus hemorrhagic shock (HS) (35?min), and compare its effects in CCI alone. C57BL/6 mice were divided into five groups (n?=?10/group): naïve, CCI+vehicle, CCI+glibenclamide, CCI+HS+vehicle, and CCI+HS+glibenclamide. Intravenous glibenclamide (10?min post-injury) was followed by a subcutaneous infusion for 24?h. Brain edema in injured and contralateral hemispheres was subsequently quantified (wet-dry weight). This protocol brain water (BW)?=?80.4% vehicle vs. 78.3% naïve, p?

SUBMITTER: Jha RM 

PROVIDER: S-EPMC6098411 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature