Ontology highlight
ABSTRACT:
SUBMITTER: Naeimi Kararoudi M
PROVIDER: S-EPMC6101749 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Naeimi Kararoudi Meisam M Dolatshad Hamid H Trikha Prashant P Hussain Syed-Rehan A SA Elmas Ezgi E Foltz Jennifer A JA Moseman Jena E JE Thakkar Aarohi A Nakkula Robin J RJ Lamb Margaret M Chakravarti Nitin N McLaughlin K John KJ Lee Dean A DA
Journal of visualized experiments : JoVE 20180614 136
CRISPR/Cas9 technology is accelerating genome engineering in many cell types, but so far, gene delivery and stable gene modification have been challenging in primary NK cells. For example, transgene delivery using lentiviral or retroviral transduction resulted in a limited yield of genetically-engineered NK cells due to substantial procedure-associated NK cell apoptosis. We describe here a DNA-free method for genome editing of human primary and expanded NK cells using Cas9 ribonucleoprotein comp ...[more]