Ontology highlight
ABSTRACT:
SUBMITTER: Xu Y
PROVIDER: S-EPMC6103843 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Xu Yi Y Lee Jeong-Heon JH Li Zhaoyu Z Wang Liguo L Ordog Tamas T Bailey Ryan C RC
Lab on a chip 20180801 17
The first step in chromatin-based epigenetic assays involves the fragmentation of chromatin to facilitate precise genomic localization of the associated DNA. Here, we report the development of a droplet microfluidic device that can rapidly and efficiently digest chromatin into single nucleosomes starting from whole-cell input material offering simplified and automated processing compared to conventional manual preparation. We demonstrate the digestion of chromatin from 2500-125 000 Jurkat cells ...[more]