Ontology highlight
ABSTRACT:
SUBMITTER: Cravero K
PROVIDER: S-EPMC6104457 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Cravero Karen K Medford Arielle A Pallavajjala Aparna A Canzoniero Jenna J Hunter Natasha N Chu David D Cochran Rory L RL Waters Ian I Christenson Eric S ES Kyker-Snowman Kelly K Button Berry B Cole Alex J AJ Park Ben Ho BH
Practical laboratory medicine 20180809
<h4>Background</h4>Genomic testing is often limited by the exhaustible nature of human tissue and blood samples. Here we describe biotinylated amplicon sequencing (BAmSeq), a method that allows for the creation of PCR amplicon based next-generation sequencing (NGS) libraries while retaining the original source DNA.<h4>Design and methods</h4>Biotinylated primers for different loci were designed to create NGS libraries using human genomic DNA from cell lines, plasma, and formalin-fixed paraffin em ...[more]