Leveraging multiple transcriptome assembly methods for improved gene structure annotation.
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ABSTRACT:
SUBMITTER: Venturini L
PROVIDER: S-EPMC6105091 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
Leveraging multiple transcriptome assembly methods for improved gene structure annotation.
Venturini Luca L Caim Shabhonam S Kaithakottil Gemy George GG Mapleson Daniel Lee DL Swarbreck David D
GigaScience 20180801 8
<h4>Background</h4>The performance of RNA sequencing (RNA-seq) aligners and assemblers varies greatly across different organisms and experiments, and often the optimal approach is not known beforehand.<h4>Results</h4>Here, we show that the accuracy of transcript reconstruction can be boosted by combining multiple methods, and we present a novel algorithm to integrate multiple RNA-seq assemblies into a coherent transcript annotation. Our algorithm can remove redundancies and select the best trans ...[more]
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