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Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

ABSTRACT: Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ?57 kDa cytochrome-P450 and negatively-charged ?16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.

SUBMITTER: Ravula T

PROVIDER: S-EPMC6107395 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

Ravula Thirupathi T Hardin Nathaniel Z NZ Bai Jia J Im Sang-Choul SC Waskell Lucy L Ramamoorthy Ayyalusamy A

Chemical communications (Cambridge, England) 20180801 69

Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ∼57 kDa cytochrome-P450 and negatively-charged ∼16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins. ...[more]

PMID: 30094448

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