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Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.

ABSTRACT: BACKGROUND:Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. RESULTS:Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewed as efficiently as those from wild-type (Pax7+/+) mice. CONCLUSIONS:Taken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases.

SUBMITTER: Kitajima Y

PROVIDER: S-EPMC6108100 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.

Kitajima Yasuo Y Ono Yusuke Y

Skeletal muscle 20180824 1

<h4>Background</h4>Satellite cells are residential muscle stem cells that express a paired box protein, PAX7.<h4>Results</h4>Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cell ...[more]

PMID: 30139390

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