Ontology highlight
ABSTRACT:
SUBMITTER: Mendelsohn BA
PROVIDER: S-EPMC6110572 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Mendelsohn Bryce A BA Bennett Neal K NK Darch Maxwell A MA Yu Katharine K Nguyen Mai K MK Pucciarelli Daniela D Nelson Maxine M Horlbeck Max A MA Gilbert Luke A LA Hyun William W Kampmann Martin M Nakamura Jean L JL Nakamura Ken K
PLoS biology 20180827 8
Insufficient or dysregulated energy metabolism may underlie diverse inherited and degenerative diseases, cancer, and even aging itself. ATP is the central energy carrier in cells, but critical pathways for regulating ATP levels are not systematically understood. We combined a pooled clustered regularly interspaced short palindromic repeats interference (CRISPRi) library enriched for mitochondrial genes, a fluorescent biosensor, and fluorescence-activated cell sorting (FACS) in a high-throughput ...[more]