Dataset Information

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.

ABSTRACT: Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45°C to 0.51molL-1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.

SUBMITTER: Rodrigues DR

PROVIDER: S-EPMC6112057 | biostudies-literature | 2018 Jul - Sep

REPOSITORIES: biostudies-literature

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Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.

Rodrigues Dalila Ribeiro DR Silva Aleksandro Ferreira da AFD Cavalcanti Maria Idaline Pessoa MIP Escobar Indra Elena Costa IEC Fraiz Ana Carla Resende ACR Ribeiro Paula Rose de Almeida PRA Ferreira Neto Reginaldo Alves RA Freitas Ana Dolores Santiago de ADS Fernandes-Júnior Paulo Ivan PI

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20180202 3

Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequence ...[more]

PMID: 29426665

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Project description:Inhibitors of peptidases isolated from leguminous seeds have been studied for their pharmacological properties. The present study focused on purification, biochemical characterization and anti-inflammatory and anticoagulant evaluation of a novel Kunitz trypsin inhibitor from Erythrina velutina seeds (EvTI). Trypsin inhibitors were purified by ammonium sulfate (30-60%), fractionation followed by Trypsin-Sepharose affinity chromatography and reversed-phase high performance liquid chromatography. The purified inhibitor showed molecular mass of 19,210.48 Da. Furthermore, a second isoform with 19,228.16 Da was also observed. The inhibitor that showed highest trypsin specificity and enhanced recovery yield was named EvTI (P2) and was selected for further analysis. The EvTI peptide fragments, generated by trypsin and pepsin digestion, were further analyzed by MALDI-ToF-ToF mass spectrometry, allowing a partial primary structure elucidation. EvTI exhibited inhibitory activity against trypsin with IC50 of 2.2×10(-8) mol.L(-1) and constant inhibition (Ki) of 1.0×10(-8) mol.L(-1), by a non-competitive mechanism. In addition to inhibit the activity of trypsin, EvTI also inhibited factor Xa and neutrophil elastase, but do not inhibit thrombin, chymotrypsin or peptidase 3. EvTI was investigated for its anti-inflammatory and anti-coagulant properties. Firstly, EvTI showed no cytotoxic effect on human peripheral blood cells. Nevertheless, the inhibitor was able to prolong the clotting time in a dose-dependent manner by using in vitro and in vivo models. Due to anti-inflammatory and anticoagulant EvTI properties, two sepsis models were here challenged. EvTI inhibited leukocyte migration and specifically acted by inhibiting TNF-α release and stimulating IFN-α and IL-12 synthesis. The data presented clearly contribute to a better understanding of the use of Kunitz inhibitors in sepsis as a bioactive agent capable of interfering in blood coagulation and inflammation.

Project description:Global climate change alters the dynamic of natural ecosystems and directly affects species distributions, persistence and diversity. The impacts of climate change may lead to dramatic changes in biotic interactions, such as pollination and seed dispersal. Life history traits are extremely important to consider the vulnerability of a species to climate change, producing more robust models than those based primarily on species distributions. Here, we hypothesized that rising temperatures and aridity will reduce suitable habitats for the endemic flora of the Caatinga, the most diverse dry tropical forest on Earth. Specifically, species with specialized reproductive traits (e.g. vertebrate pollination, biotic dispersal, obligatory cross-pollination) should be more affected by climate change than those with generalist traits. We performed two ecological niche models (current and future) to simulate the effects of climate change on the distribution area of endemic species in relation to life-history traits. We used the MIROC-ESM and CCSM4 models for both intermediate (RCP4.5) and highest predicted (RCP8.5) GHG emission scenarios, with a resolution of 30' (~1 km2). Habitat with high occurrence probability (>80%) of endemic species will be reduced (up to ~10% for trees, ~13% for non-arboreous, 10-28% for species with any pollination/reproductive system), with the greatest reductions for species with specialized reproductive traits. In addition, the likely concentration of endemic plants in the extreme northeastern portion of the Caatinga, in more mesic areas, coincides with the currently most human-modified areas of the ecosystem, which combined with climate change will further contract suitable habitats of endemic species. In conclusion, plant species endemic to the Caatinga are highly vulnerable to even conservative scenarios of future climate change and may lose much of their climatic envelopes. New protected areas should be located in the northeastern portion of the Caatinga, which hosts a more favorable climate, but is currently exposed to escalating agricultural intensification.

Dataset Information

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.

Publications

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.

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