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Reprogramming human cells to naive pluripotency: how close are we?

ABSTRACT: Pluripotent stem cells (PSCs) have the potential to revolutionise biomedical science; however, while it is simple to reproducibly obtain comparable, stable cell lines in mouse, those produced from human material typically show significant variability both within and between cell lines. This is likely due to differences in the cell identity of conventional mouse and human PSCs. It is hoped that recently identified conditions to reprogram human cells to a naïve-like state will produce better PSCs resulting in reproducible experimental outcomes and more consistent differentiation protocols. In this review we discuss the latest literature on the discovery of human naïve-like stem cells and examine how similar they are to both mouse naïve cells and the preimplantation human epiblast.

SUBMITTER: Bates LE

PROVIDER: S-EPMC6112416 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Reprogramming human cells to naïve pluripotency: how close are we?

Bates Lawrence E LE Silva José Cr JC

Current opinion in genetics & development 20170629

Pluripotent stem cells (PSCs) have the potential to revolutionise biomedical science; however, while it is simple to reproducibly obtain comparable, stable cell lines in mouse, those produced from human material typically show significant variability both within and between cell lines. This is likely due to differences in the cell identity of conventional mouse and human PSCs. It is hoped that recently identified conditions to reprogram human cells to a naïve-like state will produce better PSCs ...[more]

PMID: 28668635

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