Ontology highlight
ABSTRACT:
SUBMITTER: Kunitomi A
PROVIDER: S-EPMC9701587 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Cell reports methods 20221017 11
Naive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified me ...[more]