Dataset Information
Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe.
Ontology highlight
ABSTRACT: Background
As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools.Purpose
Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen.Method
Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus.Results
Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity.Conclusion
Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions.
SUBMITTER: Zhang T
PROVIDER: S-EPMC6112774 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe.
International journal of nanomedicine 20180823
<h4>Background</h4>As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools.<h4>Purpose</h4>Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradis ...[more]