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Highly sensitive and robust peroxidase-like activity of Au-Pt core/shell nanorod-antigen conjugates for measles virus diagnosis.

ABSTRACT: BACKGROUND:As a promising candidate for artificial enzymes, catalytically active nanomaterials show several advantages over natural enzymes, such as controlled synthesis at low cost, tunability of catalytic activities, and high stability under stringent conditions. Rod-shaped Au-Pt core/shell nanoparticles (Au@Pt NRs), prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase, with potential applications in various bio-detections. The determination of measles virus is accomplished by a capture-enzyme-linked immunosorbent assay (ELISA) using Au@Pt NR-antigen conjugates. RESULTS:Based on the enhanced catalytic properties of this nanozyme probe, a linear response was observed up to 10 ng/mL measles IgM antibodies in human serum, which is 1000 times more sensitive than commercial ELISA. CONCLUSIONS:Hence, these findings provide positive proof of concept for the potential of Au@Pt NR-antigen conjugates in the development of colorimetric biosensors that are simple, robust, and cost-effective.

SUBMITTER: Long L

PROVIDER: S-EPMC5930499 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Highly sensitive and robust peroxidase-like activity of Au-Pt core/shell nanorod-antigen conjugates for measles virus diagnosis.

Long Lin L Liu Jianbo J Lu Kaishun K Zhang Tao T Xie Yunqing Y Ji Yinglu Y Wu Xiaochun X

Journal of nanobiotechnology 20180502 1

<h4>Background</h4>As a promising candidate for artificial enzymes, catalytically active nanomaterials show several advantages over natural enzymes, such as controlled synthesis at low cost, tunability of catalytic activities, and high stability under stringent conditions. Rod-shaped Au-Pt core/shell nanoparticles (Au@Pt NRs), prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase, with potential applica ...[more]

PMID: 29720232

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Project description:Integration of plasmonic nanoantennas with catalytically active reactors in deliberately designed hybrid supra-nanostructures creates a dual-functional materials platform, based upon which precise modulation of catalytic reaction kinetics becomes accomplishable through optical excitations of plasmon resonances. Here, we have developed a multistep synthetic approach that enables us to assemble colloidal Au@C/Pt core@shell/satellite supra-nanostructures, in which the Au core functions as a light-harvesting plasmonic nanoantenna, the Pt satellites act as catalytically active reactors, and the C shell serves as a nanoscale dielectric spacer separating the reactors from the antenna, respectively. By adjusting several synthetic parameters, the size of the Au core, the thickness of the C shell, and the surface coverage of Pt satellites can all be tuned independently. Choosing Pt-catalyzed cascade oxidation of 3,3',5,5'-tetramethylbenzidine in an aerobic aqueous environment as a model reaction, we have systematically studied the detailed kinetic features of the catalytic reactions both in the dark and under visible light illumination over a broad range of reaction conditions, which sheds light on the interplay between plasmonic and catalytic effects in these antenna-reactor nanohybrids. The plasmonic antenna effect can be effectively harnessed to kinetically modulate multiple crucial steps during the cascade reactions, benefiting from plasmon-enhanced interband electronic transitions in the Pt satellites and plasmon-enhanced intramolecular electronic excitations in chromogenic intermediate species. In addition to the plasmonic antenna effect, photothermal transduction derived from plasmonic excitations can also provide significant contributions to the kinetic enhancements under visible light illumination. The knowledge gained from this work serves as important guiding principles for rational design and structural optimization of plasmonic antenna-reactor hybrid nanomaterials, endowing us with enhanced capabilities to kinetically modulate targeted catalytic/photocatalytic molecule-transforming processes through light illumination.

Dataset Information

Highly sensitive and robust peroxidase-like activity of Au-Pt core/shell nanorod-antigen conjugates for measles virus diagnosis.

Publications

Highly sensitive and robust peroxidase-like activity of Au-Pt core/shell nanorod-antigen conjugates for measles virus diagnosis.

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