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Megahertz data collection from protein microcrystals at an X-ray free-electron laser.


ABSTRACT: X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120?Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128?MHz X-ray pulse trains at 10?Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15??m XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.

SUBMITTER: Grunbein ML 

PROVIDER: S-EPMC6113309 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Megahertz data collection from protein microcrystals at an X-ray free-electron laser.

Grünbein Marie Luise ML   Bielecki Johan J   Gorel Alexander A   Stricker Miriam M   Bean Richard R   Cammarata Marco M   Dörner Katerina K   Fröhlich Lars L   Hartmann Elisabeth E   Hauf Steffen S   Hilpert Mario M   Kim Yoonhee Y   Kloos Marco M   Letrun Romain R   Messerschmidt Marc M   Mills Grant G   Nass Kovacs Gabriela G   Ramilli Marco M   Roome Christopher M CM   Sato Tokushi T   Scholz Matthias M   Sliwa Michel M   Sztuk-Dambietz Jolanta J   Weik Martin M   Weinhausen Britta B   Al-Qudami Nasser N   Boukhelef Djelloul D   Brockhauser Sandor S   Ehsan Wajid W   Emons Moritz M   Esenov Sergey S   Fangohr Hans H   Kaukher Alexander A   Kluyver Thomas T   Lederer Max M   Maia Luis L   Manetti Maurizio M   Michelat Thomas T   Münnich Astrid A   Pallas Florent F   Palmer Guido G   Previtali Gianpietro G   Raab Natascha N   Silenzi Alessandro A   Szuba Janusz J   Venkatesan Sandhya S   Wrona Krzysztof K   Zhu Jun J   Doak R Bruce RB   Shoeman Robert L RL   Foucar Lutz L   Colletier Jacques-Philippe JP   Mancuso Adrian P AP   Barends Thomas R M TRM   Stan Claudiu A CA   Schlichting Ilme I  

Nature communications 20180828 1


X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caus  ...[more]

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