Ontology highlight
ABSTRACT:
SUBMITTER: Shi WW
PROVIDER: S-EPMC6115768 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Shi Wei-Wei WW Wong Kam-Bo KB Shaw Pang-Chui PC
Toxins 20180820 8
Trichosanthin (TCS) is an RNA <i>N</i>-glycosidase that depurinates adenine-4324 in the conserved α-sarcin/ricin loop (α-SRL) of rat 28 S ribosomal RNA (rRNA). TCS has only one chain, and is classified as type 1 ribosome-inactivating protein (RIP). Our structural studies revealed that TCS consists of two domains, with five conserved catalytic residues Tyr70, Tyr111, Glu160, Arg163 and Phe192 at the active cleft formed between them. We also found that the structural requirements of TCS to interac ...[more]