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Focusing quorum sensing signalling by nano-magnetic assembly.


ABSTRACT: Quorum sensing (QS) exists widely among bacteria, enabling a transition to multicellular behaviour after bacterial populations reach a particular density. The coordination of multicellularity enables biotechnological application, dissolution of biofilms, coordination of virulence, and so forth. Here, a method to elicit and subsequently disperse multicellular behaviour among QS-negative cells is developed using magnetic nanoparticle assembly. We fabricated magnetic nanoparticles (MNPs, ?5?nm) that electrostatically collect wild-type (WT) Escherichia coli BL21 cells and brings them into proximity of bioengineered E. coli [CT104 (W3110 lsrFG- luxS- pCT6?+?pET-DsRed)] reporter cells that exhibit a QS response after receiving autoinducer-2 (AI-2). By shortening the distance between WT and reporter cells (e.g., increasing local available AI-2 concentrations), the QS response signalling was amplified four-fold compared to that in native conditions without assembly. This study suggests potential applications in facilitating intercellular communication and modulating multicellular behaviours based on user-specified designs.

SUBMITTER: Guan Y 

PROVIDER: S-EPMC6117212 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Focusing quorum sensing signalling by nano-magnetic assembly.

Guan Yongguang Y   Tsao Chen-Yu CY   Quan David N DN   Li Ying Y   Mei Lei L   Song Yingying Y   Zhang Boce B   Liu Yi Y   Payne Gregory F GF   Bentley William E WE   Wang Qin Q  

Environmental microbiology 20180701 7


Quorum sensing (QS) exists widely among bacteria, enabling a transition to multicellular behaviour after bacterial populations reach a particular density. The coordination of multicellularity enables biotechnological application, dissolution of biofilms, coordination of virulence, and so forth. Here, a method to elicit and subsequently disperse multicellular behaviour among QS-negative cells is developed using magnetic nanoparticle assembly. We fabricated magnetic nanoparticles (MNPs, ∼5 nm) tha  ...[more]

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