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Magnetic immobilization of a quorum sensing signal hydrolase, AiiA.

ABSTRACT: Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3 O4 -SiO2 iron beads and was confirmed via SDS-PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6-HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.

SUBMITTER: Wang L

PROVIDER: S-EPMC6692522 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Magnetic immobilization of a quorum sensing signal hydrolase, AiiA.

Wang Lin L Xu Haixing H Liu Zewen Z Sun Taolei T Yuan Chengqing C Yang Ying Y Guo Junhui J Xie Hao H

MicrobiologyOpen 20190214 8

Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3 O4 -SiO2 iron beads and was confirmed via SDS-PAGE, zeta potential measurement, FTIR sp ...[more]

PMID: 30767416

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