Ontology highlight
ABSTRACT:
SUBMITTER: Sakatani Y
PROVIDER: S-EPMC6117322 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Sakatani Yoshihiro Y Yomo Tetsuya T Ichihashi Norikazu N
Scientific reports 20180830 1
A major challenge in constructing artificial cells is the establishment of a recursive genome replication system coupled with gene expression from the genome itself. One of the simplest schemes of recursive DNA replication is the rolling-circle replication of a circular DNA coupled with recombination. In this study, we attempted to develop a replication system based on this scheme using self-encoded phi29 DNA polymerase and externally supplied Cre recombinase. We first identified that DNA polyme ...[more]