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A Fully Automated Continuous-Flow Platform for Fluorescence Quenching Studies and Stern-Volmer Analysis.

ABSTRACT: Herein, we report the first fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis. All the components of the platform were automated and controlled by a self-written Python script. A user-friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern-Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.

SUBMITTER: Kuijpers KPL

PROVIDER: S-EPMC6120497 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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A Fully Automated Continuous-Flow Platform for Fluorescence Quenching Studies and Stern-Volmer Analysis.

Kuijpers Koen P L KPL Bottecchia Cecilia C Cambié Dario D Drummen Koen K König Niels J NJ Noël Timothy T

Angewandte Chemie (International ed. in English) 20180727 35

Herein, we report the first fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis. All the components of the platform were automated and controlled by a self-written Python script. A user-friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern-Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a ...[more]

PMID: 29992682

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