Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC6123492 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Chen Jiayi J Liu Li L You Chenjiang C Gu Jiaqi J Ruan Wenjie W Zhang Lu L Gan Jianhua J Cao Chunyang C Huang Ying Y Chen Xuemei X Ma Jinbiao J
Nature communications 20180904 1
A family of DEDDh 3'→5' exonucleases known as Small RNA Degrading Nucleases (SDNs) initiates the turnover of ARGONAUTE1 (AGO1)-bound microRNAs in Arabidopsis by trimming their 3' ends. Here, we report the crystal structure of Arabidopsis SDN1 (residues 2-300) in complex with a 9 nucleotide single-stranded RNA substrate, revealing that the DEDDh domain forms rigid interactions with the N-terminal domain and binds 4 nucleotides from the 3' end of the RNA via its catalytic pocket. Structural and bi ...[more]