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TEM-184, a Novel TEM-Derived Extended-Spectrum ?-Lactamase with Enhanced Activity against Aztreonam.

ABSTRACT: TEM-184, a novel TEM-derived extended-spectrum ?-lactamase (ESBL), was isolated from an Escherichia coli ST354 clinical strain. Compared to TEM-1, TEM-184 contains the mutations Q6K, E104K, I127V, R164S, and M182T. Kinetic analysis of this enzyme revealed extended-spectrum activity against aztreonam in particular. TEM-184 was also susceptible to inhibitors, including clavulanic acid, tazobactam, and avibactam.

SUBMITTER: Piccirilli A

PROVIDER: S-EPMC6125571 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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TEM-184, a Novel TEM-Derived Extended-Spectrum β-Lactamase with Enhanced Activity against Aztreonam.

Piccirilli Alessandra A Perilli Mariagrazia M Amicosante Gianfranco G Conte Viola V Tascini Carlo C Rossolini Gian Maria GM Giani Tommaso T

Antimicrobial agents and chemotherapy 20180827 9

TEM-184, a novel TEM-derived extended-spectrum β-lactamase (ESBL), was isolated from an <i>Escherichia coli</i> ST354 clinical strain. Compared to TEM-1, TEM-184 contains the mutations Q6K, E104K, I127V, R164S, and M182T. Kinetic analysis of this enzyme revealed extended-spectrum activity against aztreonam in particular. TEM-184 was also susceptible to inhibitors, including clavulanic acid, tazobactam, and avibactam. ...[more]

PMID: 29941651

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