Ontology highlight
ABSTRACT:
SUBMITTER: Chung M
PROVIDER: S-EPMC6127098 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Chung Matthew M Teigen Laura L Liu Hong H Libro Silvia S Shetty Amol A Kumar Nikhil N Zhao Xuechu X Bromley Robin E RE Tallon Luke J LJ Sadzewicz Lisa L Fraser Claire M CM Rasko David A DA Filler Scott G SG Foster Jeremy M JM Michalski Michelle L ML Bruno Vincent M VM Dunning Hotopp Julie C JC
Scientific reports 20180906 1
Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared the standard enrichment of bacterial and eukaryotic mRNA to a targeted enrichment using an Agilent SureSelect (AgSS) capture for Brugia malayi, Aspergillus fumigatus, and the Wolbachia endosymbiont of B. malayi (wBm). Without introducing significant systematic bias, the AgSS quantitatively enriched samples, resulting in more reads mapping to the target organism. The AgSS-enriched libra ...[more]