Dataset Information

Radon signals in geological (natural) geogas and in a simultaneous enhanced confined mode simulation experiment.

ABSTRACT: An enhanced confined mode (ECM) radon simulation experiment, tested in the laboratory in Jerusalem, was relocated to a subsurface geophysical observatory located 400?km apart, at a depth of 150?m and with a stable temperature. Five gamma sensors are placed around the ECM canister and lead shielding minimizes the influence of natural local gamma radiation. Simultaneous measurement of the geological radon and from radon in the ECM system indicates that the temporal variation of gamma radiation from radon in the ECM system contains annual, multi-day and daily signals, that correspond to signals in the local geological radon. This implies that a common external driver influences the radiation pattern of the geological radon and from radon inside the ECM canister. Once activated at BGO the typical variation pattern of the experimental system occurring in the laboratory changed to that occurring at the observatory. This is interpreted to indicate that the overall style of the temporal patterns of radiation from radon is site dependent. The outcome of this investigation conforms and further substantiates the recent suggestion that a component in solar radiation is driving the annual and daily periodic components in the variation of radon. New geophysical research potential is indicated.

SUBMITTER: Steinitz G

PROVIDER: S-EPMC6127392 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Radon signals in geological (natural) geogas and in a simultaneous enhanced confined mode simulation experiment.

Steinitz Gideon G Kotlarsky Peter P Piatibratova Oksana O

Proceedings. Mathematical, physical, and engineering sciences 20180801 2216

An enhanced confined mode (ECM) radon simulation experiment, tested in the laboratory in Jerusalem, was relocated to a subsurface geophysical observatory located 400 km apart, at a depth of 150 m and with a stable temperature. Five gamma sensors are placed around the ECM canister and lead shielding minimizes the influence of natural local gamma radiation. Simultaneous measurement of the geological radon and from radon in the ECM system indicates that the temporal variation of gamma radiation fro ...[more]

PMID: 30220864

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Project description:The most widely-used method for detecting genome-wide protein-DNA interactions is chromatin immunoprecipitation on tiling microarrays, commonly known as ChIP-chip. Here, we conducted the first objective analysis of tiling array platforms and analysis algorithms in a simulated ChIP-chip experiment. Mixtures of human genomic DNA and "spike-ins" comprised of nearly 100 human sequences at various concentrations were hybridized to four tiling array platforms by eight independent groups. Blind to the number of spike-ins, their locations, and the range of concentrations, each group made predictions of the spike-in locations. All commercial tiling array platforms performed well, although each platform and analysis algorithm had distinct performance and cost characteristics. Simple sequence repeats and genome redundancy tend to result in false positives on oligonucleotide platforms. The spike-in DNA samples and the resulting array data presented here provide a stable benchmark against which future ChIP platforms, protocol improvements, and analysis methods can be evaluated. Keywords: chip-ChIP simulation For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each replicate array, we labeled 1Âµg of spike-in sample and 1Âµg of control sample. The Bioprime Plus Array CGH labeling Module (Invitrogen Catolog number 18095-014) was used. The spike-in was labeled with the red channel dye (Alexa Fluor-647) and the control was labeled with the green channel dye (Alexa Fluor-555) in two of the replicates. A dye swap was performed for the third replicate. These samples were then competitively hybridized to three replicate 244k arrays from Agilent, (AMADID 25150451). We hybridized the samples to the arrays using the Agilent Array CGH protocol, with some modifications. Briefly, labeled DNA was combined with Cot-1 DNA, blocking reagent, and hybridization buffer. The hybridizations were carried out at 60Â°C. The arrays were scanned using an Agilent dual laser scanner, and the images were processed using Agilent Feature Extraction Software.

Dataset Information

Radon signals in geological (natural) geogas and in a simultaneous enhanced confined mode simulation experiment.

Publications

Radon signals in geological (natural) geogas and in a simultaneous enhanced confined mode simulation experiment.

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