Ontology highlight
ABSTRACT:
SUBMITTER: Moghaddas Sani H
PROVIDER: S-EPMC6128974 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Moghaddas Sani Hakimeh H Hamzeh-Mivehroud Maryam M Silva Ana P AP Walshe James L JL Mohammadi S Abolghasem SA Rahbar-Shahrouziasl Mahdyieh M Abbasi Milad M Jamshidi Omid O Low Jason Kk JK Dastmalchi Siavoush S Mackay Joel P JP
BioImpacts : BI 20180128 3
<i><b>Introduction:</b></i> DOF proteins are a family of plant-specific transcription factors with a conserved zinc finger (ZF) DNA-binding domain. Although several studies have demonstrated their specific DNA binding, quantitative affinity data is not available for the binding of DOF domains to their binding sites. <i><b>Methods:</b></i> ZF domains of DOF2.1, DOF3.4, and DOF5.8 from <i>Arabidopsis thaliana</i> were expressed and purified. Their DNA binding affinities were assessed using gel ret ...[more]